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KMID : 0364820200560030269
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Korean Journal of Microbiology
2020 Volume.56 No. 3 p.269 ~ p.276
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Distribution of antimicrobial resistance genes in enterotoxigenic Escherichia coli isolated from diarrheic pigs
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Park Su-Jin
Ham Hee-Jin
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Abstract
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The overuse of antibiotics in animal husbandry has contributed to the emergence and dissemination of antibiotic resistance genes. The objective of this study was to determine the distribution of antimicrobial resistance genes for streptomycin, tetracycline, and sulfonamide in enterotoxigenic Escherichia coli (ETEC) isolated from diarrheic pigs. A total of 1,444 ETEC isolates were collected and examined by the antimicrobial susceptibility test and PCR test for the presence of antimicrobial resistance genes for streptomycin, tetracycline, and sulfonamide, as well as the integrase gene (int1) from class I integrons. The highest antimicrobial resistance rates were found for tetracycline (95.5%; 1,379 isolates), followed by sulfonamide (92.8%; 1,340 isolates) and streptomycin (85.0%; 1,227 isolates). Streptomycin resistance genes (aadA [69.7%; 1,007 isolates], strA [61.0%; 881 isolates], and strB [58.4%, 844 isolates]) were the most prevalent in ETEC strains followed by tetracycline (tetA [50.7%; 732 isolates] and tetB [26.9%; 388 isolates]) and sulfonamide (sul1 [35.2%; 508 isolates], sul2 [40.9%; 590 isolates], and sul3 [36.6%; 528 isolates]) resistant genes. The co-presence of int1 and aadA genes was the most common combination (47.0%; 678 isolates) found. Statistical analysis showed a significant association between resistance to an antimicrobial agent and having a resistance gene (P < 0.01). This study highlights the increase of antimicrobial resistance genes for streptomycin, tetracycline, and sulfonamide in ETEC strains isolated from pigs.
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KEYWORD
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Escherichia coli, antimicrobial resistance gene, antimicrobial susceptibility, pig
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